The analysis of gene expression is being studied using microarray, quantitative-realtime PCR, RNA-Seq, and Nanostring. These resources include:
Comprehensive analysis of gene expression during axolotl limb regeneration.
The following database shows temporal gene expression profiles from the largest microarray experiment ever performed for limb regeneration. Ten replicate Amby002 GeneChips (Huggins et al., 2012) were used to estimate gene expression for 20 different time points during limb regeneration. Two different amputation injuries were performed: amputation through the stylopod and zeugopod of the forelimb. One millimeter of the distal-most tissue of regenerating limbs was sampled for RNA isolation and microarray analysis. Overall, the database reports 8,000,000 estimates of gene expression during the first 28 days of limb regeneration.
Gene expression during the first 48 hours of axolotl embryo tail regeneration.
The following database shows temporal gene expression profiles from an RNA-Seq experiment that quantified transcript abundance at the time of amputation and at four post-amputation timepoints: 6, 12, 24, and 48 hours post-amputation. Single cell and epigenetic data will soon be made available for these timepoints to further enable the axolotl embryo tail regeneration model.
Coming soon!
Recent RNA-Seq publications
Baddar NW, Dwaraka VB, Ponomareva LV, Thorson JS, Voss SR. 2021. Chemical genetics of regeneration: contrasting temporal effects of CoCl2 on axolotl tail regeneration. In Press, Developmental Dynamics.
Rodgers AK, Smith JJ, & Voss SR. 2020. Identification of immune and non-immune cells in regenerating axolotl limbs by single-cell sequencing. Experimental Cell Research 394:112149.
Voss SR, Ponomareva LV, Dwaraka VB, Pardue KE, Baddar NW, Rodgers AK, Woodcock MR, Qiu Q, Crowner A, Blichmann D, Khatri S, Thorson JS. 2019. HDAC regulates transcription at the outset of axolotl tail regeneration. Scientific Reports 9:6751.
Dwaraka VB, Smith JJ, Woodcock MR, Voss SR. 2018. Comparative transcriptomics of limb regeneration: Identification of conserved expression changes among three species of Ambystoma. Genomics 111:1216-1225.
Archival microarray datasets
Amby001: A custom Affymetrix GeneChip© with approximately 4500 perfect and mismatch probesets. Library Files Probe Annotation File (Original) Probe Annotations (Updated)
Amby002: A custom Affymetrix GeneChip© with approximately 20,000 perfect probesets. For more information, contact your local Affymetrix representative with the array name (AMBY_002a520748F) and part number - 520748. Library Files Probe Annotation File
Page RB, Boley MA, Kump DK, Voss SR. 2013. Genomics of a metamorphic timing QTL: met1 maps to a unique genomic position and regulates morph and species-specific patterns of brain transcription. Genome Biology and Evolution, In Press. Download .CEL files
Huggins P, Johnson CK, Schoergendorfer A, Putta S, Bathke AC. Stromberg AJ, Voss SR. 2011. Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum). Comparative Biochemistry and Physiology, Part C. doi:10.1016/j.cbpc.2011.03.006. Download .CEL files
Page RB, Boley MA, Smith JJ, Putta S, Voss SR. 2010. Microarray analysis of a salamander hopeful monster reveals transcriptional signatures of paedomorphic brain development. BMC Evol Biol, 10:199. Download .CEL files
Monaghan JR, Epp LG, Putta S, Page RB, Walker JA, Beachy CK, Zhu W, Pao GM, Verma IM, Hunter T, Bryant SV,Gardiner DM, Harkins TT, Voss SR. 2009. Microarray and cDNA sequence analysis of transcription during nerve-dependent limb regeneration.BMC Biology 7:1. Download .CEL files
Cotter JD, Storfer A, Page RB, Beachy CK, Voss SR. 2008. Transcriptional response of Mexican axolotls to Ambystoma tigrinum virus (ATV) infection.,BMC Genomics 9:493 Download .CEL files
Page RB, Voss SR, Samuels AK, Smith JJ, Putta S, Beachy CK. 2008. Effect of thyroid hormone concentration on the transcriptional response underlying induced metamorphosis in the Mexican axolotl, BMC Genomics 9:78 Download .CEL files
Page RB, Monaghan JR, Samuels AK, Smith JJ, Beacy CK, Voss, SR. 2007. Microarray analysis identifies keratin loci as sensitive biomarkers for thyroid hormone disruption in the salamander Ambystoma mexicanum. Comparative Biochemistry and Physiology, Part C 145:15-27 Download .CEL files
Monaghan JR, Walker JA, Page RB, Putta S, Beachy CK, Voss SR. 2007. Early gene expression during natural spinal cord regeneration in the salamander Ambystoma mexicanum. Journal of Neurochemistry 101:27-40. Download .CEL files